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Evidence is presented for an inhibitory action of the bone marrow adherent cell layer on erythropoiesis in a case of pure red cell aplasia. Cultures of the patient's bone marrow with autologous and normal plasma showed normal numbers of CFU-C, but total absence of BFU-E. There was no inhibition of BFU-E when normal bone marrow was cultured with the patient's plasma. T-cell depletion of the patient's bone marrow did not affect these results, but after removal of the adherent cells, normal numbers of BFU-E were cultured. These observations suggest that the adherent cells, which are predominantly macrophages, were responsible for the inhibitory action on erythropoiesis.

Original publication

DOI

10.1111/j.1365-2257.1984.tb00527.x

Type

Journal article

Journal

Clin Lab Haematol

Publication Date

1984

Volume

6

Pages

61 - 67

Keywords

Aged, Anemia, Aplastic, Bone Marrow, Colony-Forming Units Assay, Erythropoiesis, Humans, Immune Adherence Reaction, Macrophages, Male