Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

<ns4:p><ns4:bold>Background:</ns4:bold> The leukaemia-derived Jurkat E6.1 cell line has been used as a model T cell in the study of many aspects of T cell biology, most notably activation in response to T cell receptor (TCR) engagement.</ns4:p><ns4:p> <ns4:bold>Methods:</ns4:bold> We present whole-transcriptome RNA-Sequencing data for Jurkat E6.1 cells in the resting state and two hours post-activation via TCR and CD28. We compare early transcriptional responses in the presence and absence of the chemokines CXCL12 and CCL19, and perform a basic comparison between observed transcriptional responses in Jurkat E6.1 cells and those in primary human T cells using publicly deposited data.</ns4:p><ns4:p> <ns4:bold>Results:</ns4:bold> Jurkat E6.1 cells have many of the hallmarks of standard T cell transcriptional responses to activation, but lack most of the depth of responses in primary cells.</ns4:p><ns4:p> <ns4:bold>Conclusions:</ns4:bold> These data indicate that Jurkat E6.1 cells hence represent only a highly simplified model of early T cell transcriptional responses.</ns4:p>

Original publication

DOI

10.12688/wellcomeopenres.15748.1

Type

Journal article

Journal

Wellcome Open Research

Publisher

F1000 Research Ltd

Publication Date

03/03/2020

Volume

5

Pages

42 - 42